Designing degenerate primers

Andrea webmaster at bioinformatik.de
Tue Sep 14 01:39:32 EST 2004


Hi Tome,

> I am sure it must be easy to do this with some kind of application 
> somewhere out there! Make a multiple alignment, save the consensus 
> sequence and directly use it for primer design! Or am I missing 
> something obvious?

Try this:

CODEHOP: COnsensus-DEgenerate Hybrid Oligonucleotide Primers
http://blocks.fhcrc.org/blocks/codehop.html

  Andrea



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