methylation

Peter Ellis pjie2 at cam.ac.uk
Wed Sep 15 03:09:36 EST 2004


oliver.schmidt at gen.bio.uni-giessen.de wrote:
>I have a 20aa peptide which is subject to methylation through an unknown
>methyltransferase.
>There are various positions in this peptide  which are possible targets for
>methylation.
>Does anyone know a method to identify which aminoacid is methylated in a
>20aa peptide of known sequence?
>(Mutation of the possible target sites would be really expensive...)
>Is it possible to check this with MALDI-TOF or is there another way for such
>a small peptide?

Peptidases that cleave at specific sites, then fractionate on a gel and 
look for size shifts of the bands?  As you can probably tell, I work 
with DNA...

Peter



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