cycloheximide question

[Kyle Legate]

Marta Rubio-Texeira wrote:
> Does anybody know of a good cycloheximide concentration to inhibit
> translation but not membrane trafficking? Is 1.5 micrograms/ml enough
> to inhibit translation in yeast cells?
>
> ---
I used to use it at 1 mM to inhibit in vitro translation using reticulocyte 
lysate and it didn't inhibit membrane targeting, though I don't know about 
trafficking. Of course, if you inhibit translation for long enough it will 
affect membrane trafficking.