looking for commercially-available high MW protein
Dr Engelbert Buxbaum
engelbert_buxbaum at hotmail.com
Thu Sep 23 05:35:21 EST 2004
peng.zhixiang at uvm.edu wrote:
> I am working with a protein which shows an approximately 470 kDa band
> in Western blotting .My problem is, I do not know if there is a
> protein around 450~500 kDa that are commercially available. Does
> anybody know such protein standards or a purified protein, ideally of
> around 470 kDa, which is supplied by manufacturers?
> Thanks in advance for advice!
Most proteins in that MW-range are multimers and can not be used in
denaturing techzniques like SDS-PAGE. However, I darkly recall seeing
high-MW standards made from chemically cross-linked serum albumine in
some catalogue or the other.
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