looking for commercially-available high MW protein
nospam at spam.dk
Thu Sep 23 12:15:40 EST 2004
Invitrogen has a new SDS page marker that goes to 500kDA
However it is very expensive, but runs nice in our gels.
"Dr Engelbert Buxbaum" <engelbert_buxbaum at hotmail.com> skrev i en meddelelse
news:ciu8ta$v9o$01$1 at news.t-online.com...
> peng.zhixiang at uvm.edu wrote:
> > Hi,
> > I am working with a protein which shows an approximately 470 kDa band
> > in Western blotting .My problem is, I do not know if there is a
> > protein around 450~500 kDa that are commercially available. Does
> > anybody know such protein standards or a purified protein, ideally of
> > around 470 kDa, which is supplied by manufacturers?
> > Thanks in advance for advice!
> Most proteins in that MW-range are multimers and can not be used in
> denaturing techzniques like SDS-PAGE. However, I darkly recall seeing
> high-MW standards made from chemically cross-linked serum albumine in
> some catalogue or the other.
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