stripping antibodies from western blots

Don Incognito imperat at hotmail.com
Sat Sep 25 23:31:35 EST 2004


"Carl Ziess" <ziess112 at yahoo.com> wrote in message news:<yEg5d.434$Rf1.419 at newssvr19.news.prodigy.com>...
> Dear all, I am in need of a good protocol for stripping only secondary
> antibody (if possible) and primary+secondary antibodies from western blots.
> This need arise from my mistakenly putting anti rabbit secondary on a blot,
> and I like to see if this blot can be reused for probing a different
> protein.  Any expert input will be appreciated. Thank you.

Assuming you haven't developed the blot, you don't need to strip it:
simply incubate with the correct secondary.

Don Incognito
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