transfection of JEG-3 cells

D.K. at
Wed Sep 29 17:53:08 EST 2004

In article <11b853f5.0409260711.df01deb at>, naomi.coslovsky at (nc) wrote:
>Need advise on transfecting JEG-3 cells:
>I didn't succeed to transfect my JEG-3 cells with the modified
>Ca-phospate method (with BES not HEPES). my regents are probably fine
>as I can transfect other cell lines I use. Any advise how to improve
>the transfection is wellcomed.

Use electroporation. It always works. 


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