Sephadex columns for nucleic acids clean up
Jill of all trades
jkellogg at avibio.com
Wed Apr 6 16:20:00 EST 2005
Way back when, a company called 5 prime -> 3 prime sold a small
sephadex column. It was used for DNA or RNA purfication subsequent to
various enzymatic reactions. The column was ready-to-use (no prep
necessary, just uncap and drain) and was designed for use in a
centrifuge/microfuge. 5 prime -> 3 prime is no longer in business and
I've been trying to find someone else who sells these things. Does
anyone know if they are still out there somewhere?
I know I can make my own. I know I can use columns that aren't
designed for a centrifuge. I know there are columns and strategies
that use something other than sephadex but right now I'm not
interested in those options, just the ready to go sephadex columns.
Thanks so much!
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