high fidelity PCR
rockyho at hotmail.com
Sat Apr 9 23:16:31 EST 2005
"Cindy" <laramore at mail.med.upenn.edu> ¼¶¼g©ó¶l¥ó·s»D:d311qr$8crc$1 at netnews.upenn.edu...
> I would like to do high fidelity PCR on cDNA and would appreciate
> suggestions from anyone with experience about the best enzyme currently
> available. Thanks.
I currently using Roche's FastStart High Fidelity Taq polymerase mix, which
is a mix of high fidelity and ordinary Taq polymerase so it gives reasonably
efficiency. My PCR products sized up to 2K for cloning experiment. Double
check for correct sequence is a must.
Of course, single enzyme PWO from the same company have the best specificity
but more expensive.
Surgery Lab, CUHK
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