high fidelity PCR

[rocky ho]

"Cindy" <laramore at mail.med.upenn.edu> ¼¶¼g©ó¶l¥ó·s»D:d311qr$8crc$1 at netnews.upenn.edu...
> Hello,
> I would like to do high fidelity PCR on cDNA and would appreciate 
> suggestions from anyone with experience about the best enzyme currently 
> available.  Thanks.
> Cindy
>
I currently using Roche's FastStart High Fidelity Taq polymerase mix, which 
is a mix of high fidelity and ordinary Taq polymerase so it gives reasonably 
efficiency. My PCR products sized up to 2K for cloning experiment.  Double 
check for correct sequence is a must.
Of course, single enzyme PWO from the same company have the best specificity 
but more expensive.

Rocky
Technician
Surgery Lab, CUHK