crude bacterial DNA prep

Michael Sullivan mlsulliv at wisc.edu
Mon Aug 8 11:58:30 EST 2005


I want to use PCR to confirm the presence of a large, low copy number  
plasmid from a bacterial strain.

Can one simply use a small amount of culture directly in a PCR  
reaction, or should one do some sort of DNA prep, even a crude one,  
prior to PCR? If the latter, does anybody have any suggestions for an  
easy prep to make PCR-quality DNA.

Thanks,

Mike
---
Michael L. Sullivan
Plant Research Molecular Geneticist
US Dairy Forage Research Center
ARS-USDA
1925 Linden Drive West
Madison, WI 53706
(608) 890-0046 (Phone)
(608) 890-0076 (FAX)



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