crude bacterial DNA prep

Michael Sullivan mlsulliv at
Mon Aug 8 11:58:30 EST 2005

I want to use PCR to confirm the presence of a large, low copy number  
plasmid from a bacterial strain.

Can one simply use a small amount of culture directly in a PCR  
reaction, or should one do some sort of DNA prep, even a crude one,  
prior to PCR? If the latter, does anybody have any suggestions for an  
easy prep to make PCR-quality DNA.


Michael L. Sullivan
Plant Research Molecular Geneticist
US Dairy Forage Research Center
1925 Linden Drive West
Madison, WI 53706
(608) 890-0046 (Phone)
(608) 890-0076 (FAX)

More information about the Methods mailing list