crude bacterial DNA prep

Louis Hom lhom at OCF.Berkeley.EDU
Mon Aug 8 22:21:27 EST 2005


We recently did some PCR analysis of colonies containing pET, which I 
think is a low copy number plasmid.  We took a small part of a colony and 
directly added it to the master mix.  You can probably do the same for 
liquid culture, but I don't know if your media contains anything 
incompatible with PCR.
-- 
______________________________________________________________________________
Lou Hom >K'93			     
lhom at ocf.berkeley.edu		
http://www.ocf.berkeley.edu/~lhom/ 	    


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