crude bacterial DNA prep

[Christian Praetorius]

Michael Sullivan <mlsulliv at wisc.edu> wrote:

>Can one simply use a small amount of culture directly in a PCR  
>reaction, or should one do some sort of DNA prep, even a crude one,  

We use 0,5µl of an overnight culture in a 25µl PCR to screen BACs (one
copy per cell) for our desired mutations. Works fine.

Christian

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