Protecting RNA in the jungle

as nospam at
Tue Aug 16 03:17:53 EST 2005

"Bo Johansen" <boj at> wrote in message 
news:dds6e1$ltt$2 at
> Hi
> I am going on a trip to collect RNA samples from flower buds in the jungle 
> of plants that cannot be cultivated. It is impossible to bring liquid 
> nitrogen on the trip, and I won't get into a lab for 2-3 weeks. What do I 
> do.
> I have thought of collecting the material in Eppendorph tubes, add 1 ml og 
> 2.5 M LiCl and crush the material with a pistil. Close the tube and leave 
> it until I get to a lab
> Or
> Collecting the material in an Eppendorph tube, add 1 ml og  4M guanidine 
> isothiocyanate, 25mM sodium citrate, pH7, 0.5% sarcosyl, 100mM 
> b-mercaptoethanolm, crush the material and leave it until I get to a lab.
> Will any of these methods leave the RNA intact or are there other methods?
> Bo

Hi Bo.
We use either Trizol (Invitrogen) or RNAlater (ambion or qiagen) for the purpose of storing 
samples for RNA extraction.
If you use Trizol it is best to keep the samples cold or frozen. I think it 
will be best NOT to crush the material until you come home.
Regards A.Salanti. Panum. 

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