Hello, I need to stain polylisine - free and conjugated with peptide. Coomasie Brillant Blue fails with free polylysine and - for my suprise - so does silver. I'm using silver staining method from Amersham Technical Manual (as it say - based on Heukeshoven and Dernick 1985) but they do not describe which aminoacids are essential for detection. Any help? Regards, Chris B.