Silver staining

Chris B. interia.pl at chris.b
Fri Aug 19 08:18:11 EST 2005


Hello,
I need to stain polylisine - free and conjugated with peptide. Coomasie
Brillant Blue fails with free polylysine and - for my suprise - so does
silver.
I'm using silver staining method from Amersham Technical Manual (as it say -
based on Heukeshoven and Dernick 1985) but they do not describe which
aminoacids are essential for detection.
Any help?
Regards,
Chris B.




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