amazonian plant DNA extraction

Lorena Quintero Barrera lquinterob at gmail.com
Mon Aug 22 09:22:06 EST 2005


Hi,

I'm trying to extract DNA from a amazonian plant, but it has been very
dificult, we assayed Doyle and Doyle, Rodgers and Bendich, and others,
and seems that we don't break the cell wall because the poor yield,
from 3 gr we obtain only 25 ng/ul, we have assayed, CTAB 3%, SDS 2%,
sarkosil, and combining CTAB ans sarkosil withoot any good result.

Please any suggestion is welcome, we really need help!, now we don't
know whaT more to use!

Lorena



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