Nucleus vs cytosol fractionation

[Dr Engelbert Buxbaum]

axexango wrote:

> Do any of you know of published protocols that has been controlled for
> ER contamination in the nuclear fraction and allow to have highly
> enriched cytosol vs nuclear fractions?

The fractionisation of cell compartments is discussed in several papers
in Meth. Enzymol. 31 (1974). However, in the end you will always have to
do assays for marker enzyme to confirm the purity of your fractions
(also described in that volume).