Western Blot Buffer Composition, why?

Kyle Legate legatekNOSPAM at hotmail.com
Wed Feb 2 16:42:31 EST 2005


axexango at hotmail.com wrote:

> 
> I know Amersham have a manual inclosed in the box with the
> western membranes that give out the reference to that question. If I
> remember correctly, PDVF membranes require methanol in their transfer
> buffer but not nitrocellulose ones. 
> 
PVDF only requires that you wet it with methanol before soaking it in 
transfer buffer, to make it hydrophilic. Once wet initially with 
methanol, no further treatment with methanol is required.



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