Fluorescence microscopy

[MMu]

Hi,
it's conventional microscopy; it seems like it is really a problem with the 
filters.
Even when there is no FITC on the slide I do see a bright green signal when 
making a colorcombine (red/green/blue) photo.


<axexango at hotmail.com> schrieb im Newsbeitrag 
news:1106966081.209949.34270 at z14g2000cwz.googlegroups.com...
> Hi
>
> jsut a question. Is this in confocal or conventional microscopy? If
> it's conventional, the signal in the nucleus might just be plasma
> membrane that is on top of the nucleus...
>
> I have never seen a shift from DAPI into green... so I can't help you
> with that...
>