Taq with RT activity ?

Oliver Berkowitz ollipolli at netscape.net
Tue Feb 15 02:07:45 EST 2005


Hi all,

does someone know if Taq Pol has some reverse transcriptase acivity ? I made
a PCR on RNA sample to check for genomic DNA contamination and got 2 bands
corresponding to genomic amplicon including intron and another band
corresponding to cDNA w/o intron. Any suggestions what happens ?

Cheers
Oliver





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