Taq with RT activity ?

Dr. Hiranya S. Roychowdhury hroychow at nmsu.edu
Tue Feb 15 11:44:20 EST 2005


In the genome, there are often copied of pseudogenes that got integrated after 
the maturation of the transcript.  It is not unusual to find such targets.

Quoting Oliver Berkowitz <ollipolli at netscape.net>:

> Hi all,
> 
> does someone know if Taq Pol has some reverse transcriptase acivity ? I
> made
> a PCR on RNA sample to check for genomic DNA contamination and got 2 bands
> corresponding to genomic amplicon including intron and another band
> corresponding to cDNA w/o intron. Any suggestions what happens ?
> 
> Cheers
> Oliver
> 
> 
> 


-- 
Hiranya S. Roychowdhury, Ph.D.
Coll. Asst. Professor,
Molecular Biology,
Dept. of Chemistry & Biochemistry
Rm# 336, Chemistry Bldg.; MSC 3MLS
New Mexico State University
Las Cruces, NM 88003
---



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