No GFP antibody for co-immunoprecipitation?
R.Jayakumar at RoswellPark.org
Wed Feb 23 09:40:40 EST 2005
Check out this website for a list of companies producing anti-GFP antibodies both polyclonal and monoclonal.
You could probably call up the company and ask them if they would be suitable for immunoprecipitation too. I will be surprised if they are not.
Best of luck.
From: owner-methods at hgmp.mrc.ac.uk
[mailto:owner-methods at hgmp.mrc.ac.uk]On Behalf Of egf_repeats
Sent: Tuesday, February 22, 2005 10:01 PM
To: methods at hgmp.mrc.ac.uk
Subject: No GFP antibody for co-immunoprecipitation?
I already made the GFP fusion for the study of a protein's
localization. Now I become interested in seeing what other proteins
(complex) it binds to. Well after a little bit research on the
internet, I realized there is actually no GFP antibody available to do
It's sort of weird that the GFP has been around for so many years and
nobody bothers to make an antibody to do co-ip with it. Anybody has an
explanation or am I wrong on this point?
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