Testing a Photobiotin labelled DNA probe. Method needed???
brilhasti at gmx.net
Mon Jan 17 09:04:53 EST 2005
> currently lies. I have exposed the DNA (5ug) to 5ug of photobiotin
> and left that under a 500W Halogen lamp (was told this would
> substitute for a sunlamp) for 15 minuites. However when completing
> the experiement I had a "no result".
Did you apply some kind of cooling to the solution while you had the lamp
It might be something as simple as the solution getting too warm.
I worked, on other experiments, with 500W halogen lamps and cooling was
necessary most of the time.
I'd second the previous post about trying to apply a UV Filter also.
Please tell us if you solve the problem.
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