about assembly heavy chain and light chain of mouse ScFv

bo yang yangbcau at hotmail.com
Tue Jul 5 23:23:58 EST 2005

Hello, everyone,

I am now doing mouse ScFv antibody by using Amersham Mouse ScFv Module Phage 
Antibody System. However, I can not link the heavy chain and light chain. I 
tried optimizing the overlapping PCR conditions such as changing template 
amount, denaturing temperature, decreasing RS primer. However, I still did 
not get ScFv fragment. I am wondering what the most important problems in 
this step are?

Your help will be greatly appreciated!


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