basic PCR question

Sarah LaMere sahlamere at
Tue Jul 12 03:30:59 EST 2005

I'm wanting to amplify a 4 kb fragment using rtPCR, and I'm just wondering what are the best PCR conditions and/or reagents people have found to use?  I know it's a very basic question, but I've never attempted to amplify a fragment that size with rtPCR, so I just want to get a decent idea of the best way to go about it.

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