sulfate containing polysaccharide question

Dr Engelbert Buxbaum engelbert_buxbaum at
Wed Jul 20 11:38:28 EST 2005

chuncent wrote:

> Hi,
> Has anyone had an experience in adjusting the pH of sulfate containing
> polysaccharides? When I adjust the pH of carrageenan and dextran
> sulfate I found that the pH would change over time. 

You will have to add some form of buffer, at least the sufate groups can
not buffer arround neutral. Some Hepes for example should do, if it is
compatible with your experiment.

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