Unwanted DNA degradation by addition of restriction enzyme buffer

Magnus Sundstrom magnus.sundstrom at gmail.com
Sun Jul 24 15:16:00 EST 2005


Hi

Anyone experienced degradation of plasmid DNA after addition of the
restriction enzyme buffer. Why is it happening and how can it be
avoided?? I get a smear on the agarose gel and it is enough to add the
buffer to get this. Very frustrating...

Thanks for your help / magnus



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