Unwanted DNA degradation by addition of restriction enzyme
Sven Even F. Borgos
svenb at biotech.ntnu.no
Mon Jul 25 02:54:58 EST 2005
Magnus Sundstrom wrote:
> Anyone experienced degradation of plasmid DNA after addition of the
> restriction enzyme buffer. Why is it happening and how can it be
> avoided?? I get a smear on the agarose gel and it is enough to add the
> buffer to get this. Very frustrating...
> Thanks for your help / magnus
My bet would be nuclease contamination of your buffer - could occur e.g.
if you happen to mess around with bare fingers (no gloves). Anyway, the
easiest thing to do is use a new tube of buffer and throw the old one
away - you probably have several of these.
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