Expression of an insoluble protein in E coli

Dr Engelbert Buxbaum engelbert_buxbaum at hotmail.com
Mon Jul 25 11:37:28 EST 2005


kevin.marley at gmail.com wrote:

> Does anyone know of a strain of E. coli that expresses human Hsp70 and
> Hsp40?  Alternatively, can I buy an expression vector (with a His tag)
> that encodes these two proteins?

The bacterial homologue to eukaryotic Hsp70/Hsp40 is DnaK/DnaJ, it's
probably easier to upregulate those than to express additional foreign
proteins.


More information about the Methods mailing list