Expression of an insoluble protein in E coli
Dr Engelbert Buxbaum
engelbert_buxbaum at hotmail.com
Mon Jul 25 11:37:28 EST 2005
kevin.marley at gmail.com wrote:
> Does anyone know of a strain of E. coli that expresses human Hsp70 and
> Hsp40? Alternatively, can I buy an expression vector (with a His tag)
> that encodes these two proteins?
The bacterial homologue to eukaryotic Hsp70/Hsp40 is DnaK/DnaJ, it's
probably easier to upregulate those than to express additional foreign
proteins.
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