basic cloning question
Sarah LaMere
sahlamere at yahoo.com
Tue Jul 26 22:03:14 EST 2005
OK, so you guys helped me amplify my 4 kb fragment from cDNA. Now I just need to clone it, and it's not cooperating.
I'm using Elongase to amplify my product, which includes a proofreading enzyme. I've been adding A overhangs to my gel product (purified with a millipore kit) and then cloning using the Invitrogen topo TA cloning kit. It's basically just not working. Any suggestions for cloning large inserts?
Thanks again,
Sarah
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