dam methylation protocol

vagos el36 at le.ac.uk
Wed Jun 1 09:51:03 EST 2005

Hello, I am looking for a protocol for methylating 10ug of pSP189
plasmid (5kb) using dam methylase. Could you please give me the exact
volume (in ul) of each constituent in the reaction?
Thanks in advance,
Evagelos Liapis

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