[Methods-and-reagents] RE: Commercial Premade SDS protein gels
dbell at fresno.ars.usda.gov
Tue Jun 7 12:52:19 EST 2005
My teaching philosophy is to have the students making & pouring their
own gels as part of the practical!
I believe that they should also be making their own buffers - teacher
supplies the recipes (i.e. 375 mM Tris.HCl, pH 8.8, 0.1% SDS) but they
need to have experience practically mauvering in a lab.
that's my 2 cents worth, be it ever so humble
**From: owner-methods at hgmp.mrc.ac.uk
**[mailto:owner-methods at hgmp.mrc.ac.uk] On Behalf Of Michael D Jones
**Sent: Tuesday, June 07, 2005 10:28 AM
**To: methods at hgmp.mrc.ac.uk
**Subject: Commercial Premade SDS protein gels
**I run a practical for MSc students, and after 9 years am tied
**of making 12% SDS PAGE gels for them!
**Can anyone recommend a UK supplier of premade SDS gels.
**I generally make 12% PAGE (375 mM Tris.HCl, pH 8.8, 0.1% SDS)
**with a 4% stack (125 mM Tris.HCl, pH 6.8, 0.1% SDS).
**The gel tanks we use are the SE 250 which take 10 x 8 cm
**gels, 0.75 mm thick.
**Any advice and comments gratefully received.
**Department of Infectious Diseases
**Division of Investigative Science
**Faculty of Medicine
**Imperial College London
**Email: m.d.jones at imperial.ac.uk
More information about the Methods