[Methods-and-reagents] Commercial Premade SDS protein gels

Michael D Jones m.d.jones at imperial.ac.uk
Tue Jun 7 12:27:58 EST 2005


Hi

I run a practical for MSc students, and after 9 years am tied of making 12%
SDS PAGE gels for them!

Can anyone recommend a UK supplier of premade SDS gels.

I generally make 12% PAGE (375 mM Tris.HCl, pH 8.8, 0.1% SDS) with a 4%
stack (125 mM Tris.HCl, pH 6.8, 0.1% SDS).

The gel tanks we use are the SE 250 which take 10 x 8 cm gels, 0.75 mm
thick.

Any advice and comments gratefully received.

Mick


Mick Jones
Department of Infectious Diseases
Division of Investigative Science
Faculty of Medicine
Imperial College London
Email:  m.d.jones at imperial.ac.uk




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