[Methods-and-reagents] Re: Commercial Premade SDS protein gels

Johanna jo-usenet at affordable-afpers.co.uk
Tue Jun 7 15:45:43 EST 2005


In article <BECB97AE.45D4%m.d.jones at imperial.ac.uk>, 
m.d.jones at imperial.ac.uk says...
> Hi
> 
> I run a practical for MSc students, and after 9 years am tied of making 12%
> SDS PAGE gels for them!
> 
> Can anyone recommend a UK supplier of premade SDS gels.
> 
> I generally make 12% PAGE (375 mM Tris.HCl, pH 8.8, 0.1% SDS) with a 4%
> stack (125 mM Tris.HCl, pH 6.8, 0.1% SDS).
> 
> The gel tanks we use are the SE 250 which take 10 x 8 cm gels, 0.75 mm
> thick.
> 
Have you tried Bio-rad?


Johanna




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