[Methods-and-reagents] RE: Quantitation of phage DNA - EtBr vs OD260

Deanne Bell dbell at fresno.ars.usda.gov
Thu Jun 9 09:56:36 EST 2005


**-----Original Message-----
**Subject: Re: Quantitation of phage DNA - EtBr vs OD260
**C Coward wrote:
**> I have prepared some bacteriophage DNA but I'm suspicious of my 
**> quantitation of it. 
*> Chris


**I always trust ethidium staining.
**
**Raymond

~*~*~*~*~*~*~*
Hi Chris
IMHO:   I think you have a very good question about differences in
abiltiy to intercalate, but I agree with Raymond and always trust my
EtBr staining over the A260/280.
HTH, Dee




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