[Methods-and-reagents] RE: Quantitation of phage DNA - EtBr vs OD260
dbell at fresno.ars.usda.gov
Thu Jun 9 09:56:36 EST 2005
**Subject: Re: Quantitation of phage DNA - EtBr vs OD260
**C Coward wrote:
**> I have prepared some bacteriophage DNA but I'm suspicious of my
**> quantitation of it.
**I always trust ethidium staining.
IMHO: I think you have a very good question about differences in
abiltiy to intercalate, but I agree with Raymond and always trust my
EtBr staining over the A260/280.
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