[Methods-and-reagents] RE: Quantitation of phage DNA - EtBr vs OD260

Deanne Bell dbell at fresno.ars.usda.gov
Thu Jun 9 13:13:26 EST 2005

** Do free nucleotides absorb at OD260? I really 
**should know that shouldn't I?

Hi again
As Raymond pointed out earlier
**>Remember that OD260 is measuring everything that absorbs at 260 nm 
**>which includes RNA and degraded DNA etc. 
I think there are things other than just nucleotides that can affect
A260/280.   Any type of purification (short of cesium) will have some
kind of contaminants.  We just don't care about them unless they
interfere with processes up stream.

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