[Methods-and-reagents] RE: Quantitation of phage DNA - EtBr vs OD260

Deanne Bell dbell at fresno.ars.usda.gov
Thu Jun 9 13:13:26 EST 2005


** Do free nucleotides absorb at OD260? I really 
**should know that shouldn't I?
**
**Chris

Hi again
As Raymond pointed out earlier
**>Remember that OD260 is measuring everything that absorbs at 260 nm 
**>which includes RNA and degraded DNA etc. 
I think there are things other than just nucleotides that can affect
A260/280.   Any type of purification (short of cesium) will have some
kind of contaminants.  We just don't care about them unless they
interfere with processes up stream.
Cheers,
Dee




More information about the Methods mailing list