[Methods-and-reagents] Re: sizing DNA fragments Macintosh

Thomas Isenbarger isen at plantpath.wisc.edu
Wed Jun 29 10:47:34 EST 2005


> Your mistake was plotting 1/distance. You are introducing a big error in 
> your fit.
>
> A reciprocal plot is nowhere near the same as a logarithm fit BTW...why 
> on earth did you do this?

I saw in a reference that this was the most accurate way to fit the 
data.  I was also looking for a balance between minimal complication in 
the fit equation and goodness of fit.

> *Always* fit to the raw data. And make sure your standards fall within 
> the resolution capabilities of the gel and that your unknowns fall 
> within the range of your standard curve.

I'll do this.  i will try tnimage and hope it's what i am looking for.  
thanks for the lead, Dima

Tom Isenbarger


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