[Methods-and-reagents] Re: sizing DNA fragments Macintosh
isen at plantpath.wisc.edu
Wed Jun 29 10:47:34 EST 2005
> Your mistake was plotting 1/distance. You are introducing a big error in
> your fit.
> A reciprocal plot is nowhere near the same as a logarithm fit BTW...why
> on earth did you do this?
I saw in a reference that this was the most accurate way to fit the
data. I was also looking for a balance between minimal complication in
the fit equation and goodness of fit.
> *Always* fit to the raw data. And make sure your standards fall within
> the resolution capabilities of the gel and that your unknowns fall
> within the range of your standard curve.
I'll do this. i will try tnimage and hope it's what i am looking for.
thanks for the lead, Dima
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