[Methods-and-reagents] Re: sizing DNA fragments Macintosh

Thomas Isenbarger isen at plantpath.wisc.edu
Wed Jun 29 10:43:20 EST 2005


In article <2xhfPQAZkowCFAGv at abuse.plus.com>,
 Duncan Clark <blackhole at abuse.plus.com> wrote:

> Historians believe that in newspost 
> <isen-07EDC1.22484028062005 at news.charter.net> on Tue, 28 Jun 2005, 
> Thomas Isenbarger <isen at plantpath.wisc.edu> penned the following 
> literary masterpiece:
> >.and my control plasmid band was 0.5 Kbp off.
> 
> When you are talking plasmid you are talking about cut linearised 
> plasmid and not uncut supercoiled. If it is uncut then I'd have thought 
> all bets are off with regards sizing programs.

it's cut


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