DNA Extraction from viscous medium

Rafael Maldonado rafamaldo at yahoo.com
Tue Mar 1 05:12:21 EST 2005


Dirk Müller wrote:
> Hi, i want to extract DNA from Sclerotium. The fungus secrets a
> polyglucan that leads to a very viscous medium. Are there any methods
> for such a case? How can i get rid of the polyglucan without loosing
> or damageing the mycelium? Chancing the medium is unfortunately no
> option...
> thank you, Dirk...

May be an extraction by the CTAB method. CTAB will remove the 
polysaccharides of your sample, while the DNA remains in solution.

-R



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