How do I lyse these cells?

D.K. dk at no.email.thankstospam.net
Tue Mar 1 21:29:40 EST 2005


In article <1109712035.245734.133490 at l41g2000cwc.googlegroups.com>, "CAS" <cs_nanae at acornhosting.net> wrote:
>Hello all,
>
>I've always had a sonicator handy, and now I find myself without one.
>
>So I'm looking for suggestions as to how to get an over-expressed
>soluble enzyme out of some E. coli BL21(DE3) cells.  No sonicator, no
>french press.
>
>I've looked at the CLONTECH kit to use with the TALON his-tag system,
>but it's expensive and my budget is tiny.  (Otherwise, I'd just buy a
>sonicator!)

Lysozyme well then add DNAse and non-ionic detergent 
(like 0.5% Triton X-100 final). Works perfectly well without stupid 
fancy-shmancy kits. 

DK



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