Difference between SOC and NZY+ Amine broth

CAS cs_nanae at acornhosting.net
Thu Mar 3 17:12:01 EST 2005


R Breton wrote:
> Hi there,
> Im using the Quikchange kit from stratagene for the first and in the
> procedure it says to use NZY amine broth, whilst the procedure for
the
> XL1 Blue Supercompetant cells says to use SOC broth. The only
> difference i can see is that SOC contains 20g/l tryptone whilst NZY
> contains 10g/l of NZ Amine mix. What is the difference between these
> two sources, as far as i can see they're both caesin digests.
>
> Many thanks and a happy new year!
> Robert Breton

To belatedly add my two cents to the mix: I typically just use LB with
the Quickchange protocol.  Works fine, and I always have some on hand.
:)




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