Difference between SOC and NZY+ Amine broth
dk at no.email.thankstospam.net
Fri Mar 4 07:58:14 EST 2005
In article <1109887921.300977.119450 at f14g2000cwb.googlegroups.com>, "CAS" <cs_nanae at acornhosting.net> wrote:
>R Breton wrote:
>> Hi there,
>> Im using the Quikchange kit from stratagene for the first and in the
>> procedure it says to use NZY amine broth, whilst the procedure for
>> XL1 Blue Supercompetant cells says to use SOC broth. The only
>> difference i can see is that SOC contains 20g/l tryptone whilst NZY
>> contains 10g/l of NZ Amine mix. What is the difference between these
>> two sources, as far as i can see they're both caesin digests.
>> Many thanks and a happy new year!
>> Robert Breton
>To belatedly add my two cents to the mix: I typically just use LB with
>the Quickchange protocol. Works fine, and I always have some on hand.
That, and any generic home-made electrocompetent cells are always
better than Stratagene's chemical ones anyway.
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