ideal medium for HepG2 cells?

D.K. dk at no.email.thankstospam.net
Fri Mar 4 20:28:11 EST 2005


In article <6.2.1.2.0.20050304204900.0275c810 at pop.gmx.net>, hubahopp at gmx.de (Wolfgang Schechinger) wrote:
>Dear all,
>
>some people grow HepG2 cells with lots of additions like Aprotinin, 
>Insulin, Transferrin, alpha Fetoprotein etc..
>
>The ATCC manual just suggests RPMI with 10% serum.
>
>When does it make sense to add all that expensive stuff?

When it's serum free medium. Serum already contains all that stuff
(or better).  Don't know about RPMI though. My memory tells me 
that all HepG2 culture I saw was in DMEM/F-12. 

DK



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