ideal medium for HepG2 cells?

Nick Theodorakis nick_theodorakis at
Fri Mar 4 21:31:42 EST 2005

On Sat, 05 Mar 2005 01:28:11 GMT, dk at (D.K.)

>In article < at>, hubahopp at (Wolfgang Schechinger) wrote:
>>Dear all,
>>some people grow HepG2 cells with lots of additions like Aprotinin, 
>>Insulin, Transferrin, alpha Fetoprotein etc..
>>The ATCC manual just suggests RPMI with 10% serum.
>>When does it make sense to add all that expensive stuff?
>When it's serum free medium. Serum already contains all that stuff
>(or better).  Don't know about RPMI though. My memory tells me 
>that all HepG2 culture I saw was in DMEM/F-12. 

I used to grow them in MEM-alpha with 10% FCS.


Nick Theodorakis
nick_theodorakis at
contact form:

More information about the Methods mailing list