Getting full-length transcripts: PCR hell

Kuei-Yang Hsiao ky.hsiao at gmail.com
Sat Mar 5 13:40:25 EST 2005


It's just my opinion!

Why don't you try to scale down the amplicons to about 1 Kb, and each
overlapping?

Then these several fragments can be pooled and re-amplified!!

Good luck!

Kuei-Yang Hsiao
Department of Physiology
National Cheng Kung University

On 5 Mar 2005 09:39:28 -0800, Emilie <C.Emilie at gmail.com> wrote:
> Hello,
> 
> -------
> Emilie
> Department of Biological Sciences, Warwick University
> c.emilie at gmail.com
>
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