Getting full-length transcripts: PCR hell

Emilie C.Emilie at gmail.com
Sun Mar 6 13:33:08 EST 2005


I tried that, but failed miserably!
This week I'm repeating this approach, plus another nested approach
using primers designed to the UTR for the first round, and start/stop
primers for the nested round.



ky.hsiao at gmail.com (Kuei-Yang Hsiao) wrote in message news:<cfab8fdd05030510333bf14051 at mail.gmail.com>...
> It's just my opinion!
> 
> Why don't you try to scale down the amplicons to about 1 Kb, and each
> overlapping?
> 
> Then these several fragments can be pooled and re-amplified!!
> 
> Good luck!
> 
> Kuei-Yang Hsiao
> Department of Physiology
> National Cheng Kung University
> 
> On 5 Mar 2005 09:39:28 -0800, Emilie <C.Emilie at gmail.com> wrote:
> > Hello,
> > 
> > -------
> > Emilie
> > Department of Biological Sciences, Warwick University
> > c.emilie at gmail.com
> >
> ---



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