Painful thrombin digestion

Nick Theodorakis nick_theodorakis at
Sat Mar 19 12:58:33 EST 2005

On Sat, 19 Mar 2005 16:45:25 +0000, ChenHA <hzhen at> wrote:

>Belle wrote:
>> So, I'm clueless about what to do at this moment. Would anyone have
>> any suggestions?
>Just a few general suggestions - 
>1) If others have got success with thrombin digest without adding
>PMSF, then why not try it?

That would seem a reasonable thing to try, since PMSF would be
expected to inhibit thrombin. You may think you washed it all out, but
why take a chance?


Nick Theodorakis
nick_theodorakis at
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