amino acid mixture

Rafael Maldonado rafamaldo at yahoo.com
Mon May 9 04:33:13 EST 2005


GysdeJongh wrote:
> "tracy" <dreamwestus at yahoo.com> wrote in message
> news:Ubree.1253$II.871 at news.itd.umich.edu...
> 
>>Does anybody know a better way to make a 20 amino acid mixture?  I weighed
>>each of them and combined them and autoclaved them, however, there is always
>>something precipitating after a few days.  Thanks,
> 
> 
> 
> Hi,
> are you sure that your mixture survived the autoclave ????
> We make 10 x stocks , steriel filter , store at -20 in small aliquotes and mix
> when needed.
> hth
> Gys
> 

Amino acids survive autoclave very well, at least all remains in good 
condition to be useful.

-R



More information about the Methods mailing list