DNA elution from agarose-gel
Jose de las Heras
josenet at tiscali.co.uk
Fri May 13 19:53:37 EST 2005
"Scooter the Mighty" <Greyguy3 at hotmail.com> wrote in message
news:1116023890.286348.98460 at g47g2000cwa.googlegroups.com...
>I used to work on an 85kb plasmid, and the ONLY way I could get it out
> of a gel was using NEB's beta-agarase enzyme. Like you, I could
> electroelute it onto a membrane but not get it off the membrane.
> If they still make beta agarase I'd give it a try.
I used to work with PACs of up to 230kb, and the only way to get them out of
the gel intact was to digest the agarose with agarase. I used Roche's
Agarase but they stopped selling it. Subsequently I've used the equivalent
from Promega (I think called AgarAce?), or that one froom NEB will be fine
too, they're all essentially the same stuff. So you could try this approach.
It's longer than using a column system like that from Qiagen... but if it
works well for 230kb, it will work for 15kb.
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