Enzyme immobilization.

Chris B. chris.b.nospam at nospam.interia.pl
Mon May 16 01:40:32 EST 2005

This may be stupid and/or simple question but I can't find answer...
Can I and how I can bind protease (pepsin, trypsin) to chitosan or sephadex?
I heard about binding enzyme to chitosan using glutaraldehyde and to
sepharose using cyanogen bromide.
Does anybody have good and cheap method to share with protein science
freshman? :)

And one more thing - what is stability of such an enzyme? After how much
time or how many use it looses activity (below "resonable" level)?

Thanks in advance...


More information about the Methods mailing list