cot assay (DNA-reassociation kinetics) on realtime pcr machine

josmarlangner at yahoo.de josmarlangner at yahoo.de
Thu May 26 03:50:10 EST 2005


Dear all,
has anyone ever performed a cot assay for DNA-reassociation kinetics on
a realtime pcr machine? Plotting SGI emission over temperature during a
low ramp cooling down phase, let's say from 95° to 50° should be all
I need. How much DNA is needed for a good signal? How much SGI is
needed (Sigma does not provide the concentration of the dye, they just
say it's 10000x). Any hints are appreciated!!!
Cheers
Josmar




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